Catalog Number - 68322-05P-2

The 68322‑05P‑2 Cogent RP PFP™ HPLC Column is designed for enhanced selectivity of halogenated, aromatic, and polar compounds, delivering improved resolution where traditional C18 phases may not fully resolve analytes.

This stationary phase utilizes a pentafluorophenyl (PFP, “F5”) ligand bonded via a four‑carbon linker to high‑purity silica with full double end‑capping, providing multiple interaction mechanisms including π‑π interactions, dipole interactions, and hydrogen bonding. These mechanisms allow for greater selectivity control and improved separation of structurally similar compounds.

The 100 Å pore size provides broad applicability across small to moderately sized molecules, making it a versatile choice for analytical and LC‑MS workflows.

50 mm Length – Fast Screening & Method Development

The 50 mm length is optimized for rapid separations and method scouting, enabling efficient evaluation of selectivity.

• Short run times for high throughput

• Efficient screening of retention behavior

• Reduced solvent consumption

✅ Use when fast method development and selectivity evaluation are priorities

Flow Rate & System Requirements (2.1 mm ID, 3 µm)

⚠️ Requires low‑flow HPLC or LC‑MS system capability

• Typical flow rate range: ~0.2–0.4 mL/min

• Optimal starting range: ~0.25–0.35 mL/min

• Higher backpressure vs 5 µm columns

• Compatible with low‑volume LC‑MS detectors

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose PFP (F5) for Selective Separations

Enhanced Retention for Halogenated Compounds

• Strong interaction with fluorinated and halogenated analytes

• Improved selectivity vs C18

Multiple Interaction Mechanisms

• π‑π interactions for aromatic compounds

• Dipole and hydrogen bonding interactions

• Greater flexibility in method development

Balanced Selectivity (100 Å Pore)

• Suitable for a wide range of analytes

• Supports both small and moderately sized molecules

Cogent RP PFP™ Advantages

Fully End‑Capped Surface

• Minimizes silanol activity

• Improves peak symmetry for polar analytes

High Efficiency (3 µm Particles)

• More efficient than 5 µm columns

• Sharper peaks and improved resolution

High Purity Silica

• Consistent performance

• Reliable reproducibility across analyses

Typical Applications

• Halogenated compound analysis

• Aromatic compound separations

• Pharmaceutical analytical workflows

• LC‑MS method development

• USP L43 method applications

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Designed for low‑flow LC‑MS and analytical HPLC systems (2.1 mm ID)

• Higher backpressure than larger particle columns

• Use 0.2 µm filtration to maintain column performance